Optimization of Moist and Oven-Dried Bacterial Cellulose Production for Functional Properties

Bacterial cellulose (BC) is a natural polymer with properties suitable for tissue engineering and possible applications in scaffold production. However, current procedures have limitations obtaining BC pellicles the desired structural, physical, mechanical properties. Thus, this study analyzed optimal culture conditions of membranes two types processing: draining oven-drying. The aim was to obtain wound dressing material. Two studies were carried out. In preliminary study, medium (100 mL) inoculated varying volumes (1, 2, 3, 4, 5 incubated statically different periods (3, 6, 9, 12, 18 days), using full factorial experimental design. Thickness, uniformity, weight, yield evaluated. optimization Box–Behnken design used. independent variables used: inoculum volume (X1: 1, fermentation period (X2: d) determine target response variables: thickness, swelling ratio, drug release, fiber diameter, tensile strength, Young’s modulus both dry moist membranes. mathematical modelling effect performed by surface methodology (RSM). obtained models validated new values confirmed all tested properties, except oven-dried BC. terms material an 5% (v/v) 16 d fermentation. While, membranes, 4% 14